Review of 4Pi Fluorescence Nanoscopy

Review of 4Pi Fluorescence Nanoscopy

Blog Article

Since the 1990s, continuous technical and scientific advances have defied the diffraction limit in microscopy and enabled three-dimensional (3D) super-resolution imaging.An important milestone in this pursuit is the Square Compact Mirror coherent utilization of two opposing objectives (4Pi geometry) and its combination with super-resolution microscopy.Herein, we review the recent progress in 4Pi nanoscopy, which provides a 3D, non-invasive, diffraction-unlimited, and isotropic resolution E-Files in transparent samples.

This review includes both the targeted and stochastic switching modalities of 4Pi nanoscopy.The schematics, principles, applications, and future potential of 4Pi nanoscopy are discussed in detail.